Free The People in the Trees by Hanya Yanagihara Page B

stomach up. You’d cut out each spleen—a tiny, savory-looking thing, richly, meatily brown and the size of a slender watermelon seed—and place each in its own Petri dish with a bit of saline. You’d have next to you a springy pile of fine wire mesh, cut into one-inch-square pieces, which you’d sterilize over a flame, and then you’d rub the spleen against it into another Petri dish to obtain a single cell suspension. Spleens, of course, are soft and pulpy, like foie gras, and you had to be careful to only brush them against the mesh; anything more vigorous and you’d find the organ smeared over your fingers, sticky and dark as fudge. You might do this a few times, or until the organ had turned liquidy; then you’d pipette some of the sauce into a tube, examine it under a microscope, and record how many cells there were per milliliter.
    The main point of these experiments was, as I’ve noted before, not only to prove that cancers were caused by viruses (note that I have not said whether cancers were caused by viruses; Smythe, whether through his own arrogance or because he had decided to make the terrible error of believing what a science writer—always an oxymoron—had written about him, seemed to have convinced himself that his theory was impregnable. His lab was not interested in proving or disproving him; Fitch and Brassard and the rest were interested only in the specifics of his supposition rather than in its inherent veracity) but also how to establish a cell culture. If you could prove that, say, Cancer X was caused by Virus Y, then all you’d have to do was create a vaccine, which would eradicate the cancer. (I’m simplifying, but not by much; this is really how they thought at the time, not just in medicine but in all the sciences: you make a bomb; you drop it on a troublesome people; the troublesome people are no more.)
    One experiment I was made to repeat involved kidneys, whose malformations were easy to identify—easier than spleens’, for example. You’d take a mouse’s kidney (a more fibrous organ than the spleen) and snip it into bits into a test tube. Then you’d take those pieces and pass them through layers of increasingly fine screens totry, again, to reduce it to a single cell layer, which would be distinguishable by its smeary quality. After that, you’d pulverize the tissue with saline and fetal calf serum nutrient—which of course helps promote growth—before placing this in a sterile bottle with a flat surface and incubating it at 37 degrees. The cells, in suspension, would then attach to the surface of the bottle, appearing in flat, starry clusters. Once you had a thriving monolayer of cells, you’d introduce a virus, inoculating the cells. After a few days, you’d then centrifuge the whole batch and remove the supernate—the noncellular part—as your vaccine.
    That was the thinking, anyway. And I have to admit, at the time this method seemed sensible, logical. Perhaps, in retrospect, a bit too sensible, a bit too logical, but it was more plausible than many of the prevailing theories of the time, although as I would learn shortly after, what is most plausible is not necessarily the most correct or worthy of the most consideration. More often it is the outlandish theory, the one that seems so improbable, that you find yourself returning to again and again, paying it an outsized share of attention largely because you find yourself intrigued by the originality of thought behind it.

    I was twenty-four; I was infecting dogs. I took syringes of various viruses and injected them into dogs’ kidneys. They were very keen on organ transplantation in those days, and so soon I was doing real surgeries, albeit on dogs, and I was able to do them unsupervised, right there in the canine lab (sometimes Parton would walk in, gaze at me dolefully, as if he’d no idea who I was and it was not his right to ask, and then shuffle out without saying a word to me). I opened up the dog and tied